Journal
PLANTA MEDICA
Volume 76, Issue 5, Pages 497-499Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-0029-1186235
Keywords
Lonicera japonica; Caprifoliaceae; nrDNA ITS region PCR-RFLP; allele-specific; diagnostic PCR
Ask authors/readers for more resources
The flower buds of Lonicera japonica Thunb. are widely used in Chinese medicine for their anti-inflammatory prosperities. Some closely related species of L. japonica which have similar morphology but weaker biological activity are also used medicinally. The discrimination of L. japonica from its adulterants is currently limited to methods of morphology and chemical fingerprinting. The analysis of sequence variation in the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) has recently become an effective method for identification of medicinal herbs. In the present study, we found that nrDNA ITS sequences differ significantly between L. japonica and the 4 tested adulterant species. We identified a distinctive site which can be digested by restriction endonuclease Eco52 I and designed a pair of allele-specific primers for diagnostic PCR on the nrDNA ITS2 region. These two techniques provide accurate, effective, and rapid approaches to distinguish L. japonica from closely related species.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available