Phytochemical investigation of Anophalis lactea

Four new compounds, 2,6 -dihydroxy-3'-(2-hydroxy-3-methyl-3-butenyl)-4'-methoxychalcone (1), 4,6-dihydroxy-7-isobutyryl-5-prenyl-2(3H)-benzofuranone (4), 7-O-(beta-D-glucopyranosyloxy)-5-hydroxy-1 (3H)-isobenzofuranone (6) and (2R,3S)-3-methyl-2-(5-oxo-2-isopropenylhexyl)-cyclopentanone (7), along with thirteen known compounds were isolated from the whole plant Anaphalis lactea. Their structures were established based on spectroscopic methods including IR, UV, MS, CD, 1 D NMR and 2 D NMR techniques. Compounds 2, 4-6 and 8-16 were tested for free-radical scavenging properties in the DPPH assays.