Journal
PLANTA
Volume 236, Issue 4, Pages 1305-1313Publisher
SPRINGER
DOI: 10.1007/s00425-012-1692-0
Keywords
Bovine papillomavirus; Codon optimization; Plant expression; Vaccine protein; Virus-like particles
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Funding
- EU PLAPROVA [KBBE-2008-227056]
- Scottish Government's Rural and Environmental Science and Analytical Services (RESAS) Division
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000CA374, BBS/E/J/00000166] Funding Source: researchfish
- BBSRC [BBS/E/J/000CA374] Funding Source: UKRI
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Bovine papillomavirus type 1 (BPV-1) is an economically important virus that induces tumourigenic pathologies in horses and cows. Given that the BPV-1 L1 major coat protein can self-assemble into highly immunogenic higher-order structures, we transiently expressed it in Nicotiana benthamiana as a prelude to producing a candidate vaccine. It was found that plant codon optimization of L1 gave higher levels of expression than its non-optimized counterpart. Following protein extraction, we obtained high yields (183 mg/kg fresh weight leaf tissue) of relatively pure L1, which had self-assembled into virus-like particles (VLPs). We found that these VLPs elicited a highly specific and strong immune response, and therefore they may have utility as a potential vaccine. This is the first report demonstrating the viable production of a candidate BPV vaccine protein in plants.
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