Journal
PLANTA
Volume 234, Issue 6, Pages 1201-1214Publisher
SPRINGER
DOI: 10.1007/s00425-011-1474-0
Keywords
Concord grape; Detoxification mechanism; Flavonol; Glucosyltransferase; Xenobiotic
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada
- Tier 1 Canada Research Chair in Plant Biotechnology
- NSERC
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Grapes berries produce and accumulate many reactive secondary metabolites, and encounter a wide range of pathogen- and human-derived xenobiotic compounds. The enzymatic glucosylation of these metabolites changes their reactivity, stability and subcellular location. Two ESTs with more than 90% nucleotide sequence identity to three full-length glucosyltransferases are expressed in several grape tissues. The full-length clones have more than 60% amino acid sequence similarity to previously characterized flavonoid 7-O-glucosyltransferases, catechin O-glucosyltransferases and anthocyanin 5-O-glucosyltransferases. In vitro, these enzymes glucosylate flavonols and the xenobiotic 2,4,5-trichlorophenol (TCP). Kinetic analysis indicates that TCP is the preferred substrate for these enzymes, while expression analysis reveals variable transcription of these genes in grape leaves, flowers and berry tissues. The in vivo role of these Vitis labrusca glucosyltransferases is discussed.
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