Journal
PLANTA
Volume 230, Issue 2, Pages 387-395Publisher
SPRINGER
DOI: 10.1007/s00425-009-0956-9
Keywords
Curcin-L; Promoter; Jatropha; Ribosome-inactivating protein
Categories
Funding
- 11th Five-Year Plan [2006BAD07A04, 2007BAD50B05]
- General Program of National Natural Science Foundation of China [30670204]
- China International Science and Technology Cooperation Project [2006DFB63400]
- Key Technology Study Project of Sichuan [05NG002-002-1]
Ask authors/readers for more resources
Ribosome-inactivating proteins (RIPs) represent a type of protein that universally inactivates the ribosome thus inhibiting protein biosynthesis. Curcin-L was a type I RIP found in Jatropha curcas L.. Its expression could be activated in leaves by treatments with abscisic acid, salicylic acid, polyethylene glycol, temperature 4, 45A degrees C and ultraviolet light. A 654 bp fragment of a 5' flanking region preceding the curcin-L gene, designated CP2, was cloned from the J. curcas genome and its expression pattern was studied via the expression of the beta-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the CP2 was leaf specific, and was able to drive the expression of the reporter gene under stress-induction conditions. Analysis of a series of 5'-deletions of the CP2 suggested that several promoter motifs were necessary to respond to environmental stresses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available