Journal
PLANTA
Volume 230, Issue 3, Pages 469-479Publisher
SPRINGER
DOI: 10.1007/s00425-009-0950-2
Keywords
Cell membrane recovery; Electroporation; Metabolic analysis; OPLS multivariate data analysis; Stress response; Wounding
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Funding
- Portuguese Foundation of Science (FCT, Portugal)
- The Royal Physiographic Society in Lund, Sweden
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Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
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