Journal
PLANT SYSTEMATICS AND EVOLUTION
Volume 300, Issue 1, Pages 113-125Publisher
SPRINGER WIEN
DOI: 10.1007/s00606-013-0864-0
Keywords
CMA/DAPI chromosome banding; FISH; Flow cytometry; Heterochromatin; Phylogeny; Solanum sect. Acanthophora
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Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Agencia FONCyT
- SECyT (Universidad Nacional de Cordoba, Argentina)
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In order to study the evolution of different cytogenetic characters in species of Solanum sect. Acanthophora in relationship to the known phylogeny for this group, the following techniques were used: CMA/DAPI chromosome banding; fluorescent in situ hybridization with probes for the 18-5.8-26S and the 5S rDNA genes in mitotic chromosomes; nuclear DNA quantification by flow cytometry. Depending on the species, 2-6 of the 12 basic chromosome pairs were identified. The heterochromatic banding patterns were shown to be species-specific. All species presented one chromosome pair bearing a 18-5.8-26S signal and one pair (rarely two) with a 5S signal, the two rDNA sites being non-syntenic. The techniques employed allowed us to establish two species groups within sect. Acanthophora: one with small, symmetric chromosomes, little heterochromatin and lower DNA content, and the other one with larger and more asymmetric chromosomes, more heterochromatin CMA(+)/DAPI(-) (associated with NOR or not) and a higher DNA content. An elevated karyotype asymmetry would be associated with a high amount of heterochromatin and a high DNA content. The trend within sect. Acanthophora would be towards a loss of heterochromatin, a reduction of chromosome size, and an increase in symmetry.
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