Journal
PLANT SCIENCE
Volume 228, Issue -, Pages 98-106Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2014.02.008
Keywords
Chloroplast transformation; Peroxidase; Antimicrobial proteins; Disease resistance; Untranslated region; Transgene expression
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Funding
- Louisiana Governor's Biotechnology Initiative
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The chloroperoxidase (cpo) gene from Pseudomonas pyrrocinia was transformed into the plastid genome (plastome) of Nicotiana tabacum var. Petit Havana and transplastomic lines were compared with a nuclear transformant for the same gene. Southern analysis confirmed integration in the plastome and western blotting confirmed the presence of the chloroperoxidase protein (CPO) in higher abundance in transplastomic plants than in cpo nuclear transformants. Northern analysis of primary plastome transformants for cpo showed 15-fold higher transcript abundance than in the nuclear transformant, yet this extent of enhancement was not observed in western blot, enzyme or bioassay, indicating a bottleneck at the post-transcriptional level. Representative plants from the two transplastomic lines showed resistance to fungal pathogens in vitro (Aspergillus flavus, Fusarium verticillioides, and Verticillium dahliae) and in planta (Alternaria alternata). Published by Elsevier Ireland Ltd.
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