Journal
PLANT SCIENCE
Volume 225, Issue -, Pages 117-129Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2014.06.006
Keywords
Hydrogen sulfide; Medicago sativa; Oxidative damage; Potassium efflux; Redox homeostasis; Salt tolerance
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Funding
- National Natural Science Foundation of China [J1210056, J1310015]
- Fundamental Research Funds for the Central Universities [KYTZ201402]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Despite the external application of hydrogen sulfide (H2S) conferring plant tolerance against various environmental cues, the physiological significance of L-cysteine desulfhydrase (L-DES)-associated endogenous H2S production involved in salt-stress signaling was poorly understood. To address this gap, the participation of in planta changes of H2S homeostasis involved in alfalfa salt tolerance was investigated. The increasing concentration of NaCl (from 50 to 300 mM) progressively caused the induction of total L-DES activity and the increase of endogenous H2S production. NaCl-triggered toxicity symptoms (175 mM), including seedling growth inhibition and lipid peroxidation, were alleviated by sodium hydrosulfide (NaHS; 100 mu M), a H2S donor, whereas aggravated by an inhibitor of L-DES or a H2S scavenger. A weaker or negative response was observed in lower or higher dose of NaHS. Further results showed that endogenous L-DES-related H2S modulated several genes/activities of antioxidant defence enzymes, and also regulated the contents of antioxidant compounds, thus counterbalancing the NaCl-induced lipid peroxidation. Moreover, H2S maintained K+/Na+ homeostasis by preventing the NaCl-triggered K+ efflux, which might be result form the impairment of SKOR expression. Together, our findings indicated that endogenous H2S homeostasis enhance salt tolerance by coupling the reestablishment of redox balance and restraining K+ efflux in alfalfa seedlings. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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