Journal
PLANT SCIENCE
Volume 225, Issue -, Pages 102-106Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2014.05.018
Keywords
Pollen germination; Pollen maturation; Sucrose phosphate synthase; Oryza sativa L
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Funding
- JSPS KAKENHI Grant [25450032]
- Grants-in-Aid for Scientific Research [12J06490, 24580030, 25450032, 14J10964] Funding Source: KAKEN
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The molecular function of an isoform of sucrose phosphate synthase (SPS) in rice, OsSPS1, was investigated using gene-disruption mutant lines generated by retrotransposon insertion. The progeny of the heterozygote of disrupted OsSPS1 (SPS1(+/-)) segregated into SPSV+/+, SPSV1(+/-), and SPS1(-/-) at a ratio of 1:1:0. This distorted segregation ratio, together with the expression of OsSPS1 in the developing pollen revealed by quantitative RT-PCR analysis and promoter-beta-glucuronidase (GUS) fusion assay, suggested that the disruption of OsSPS1 results in sterile pollen. This hypothesis was reinforced by reciprocal crosses of SPS1(+/-) plants with wild-type plants in which the disrupted OsSPS1 was not paternally transmitted to the progeny. While the pollen grains of SPS+/- plants normally accumulated starch during their development, pollen germination on the artificial media was reduced to half of that observed in the wild-type control. Overall, our data suggests that sucrose synthesis via OsSPS1 is essential in pollen germination in rice. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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