Journal
PLANT SCIENCE
Volume 207, Issue -, Pages 25-36Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2013.02.008
Keywords
Arabidopsis thaliana; Brassica napus; Elaioplast; Pollen coat; Tapetal cell; Tapetosome
Categories
Funding
- Plant Transformation Network
- Ministry of Education, Culture, Sports, Science and Technology, Japan [17051013, 19039014, 21027016]
- Japan Society for the Promotion of Science (JSPS) [23370018, 20000304]
- Japan Science and Technology Agency
- Global COE Program Advanced Systems-Biology: Designing the Biological Function
- JSPS
- Grants-in-Aid for Scientific Research [24657030, 21027016, 19039014, 17051013, 23370018] Funding Source: KAKEN
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The pollen coat covering the surface of pollen grains has many important roles for pollination. In Brassicaceae plants, the pollen coat components are synthesized and temporarily accumulated in two tapetum-specific organelles, the elaioplast and the tapetosome. Although many biochemical and electron microscopic analyses have been attempted, the structure and biogenesis of these organelles have not been fully elucidated. To resolve this problem, we performed live imaging of these organelles using two markers, FIB1a-GFP and GRP17-GFP. FIB1a is an Arabidopsis fibrillin, a structural protein of elaioplast plastoglobules. In transgenic Arabidopsis, fluorescence of FIB1a-GFP appeared in young elaioplasts, in which small plastoglobules were developing. However, the fluorescence disappeared in later stages, while enlargement of plastoglobules continued. GRP17 is an Arabidopsis oleopollenin, an oleosin-like protein in tapetosomes. Fluorescence microscopy of GRP17-GFP expressed in Arabidopsis and Brassica napus revealed that tapetosomes do not contain oleopollenin-coated vesicles but have an outer envelope, indicating that the tapetosome structure is distinct from seed oil bodies. Visualization of GRP17-GFP also demonstrated that the tapetal cells become protoplasts and migrate into locules before pollen coat formation, and provided live imaging of the foot formation between pollen grains and stigmatic papilla cells. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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