Journal
PLANT SCIENCE
Volume 187, Issue -, Pages 69-82Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2012.01.001
Keywords
Cucumber (Cucumis sativus L.); Proteome; Salicylic acid; Protein interaction network
Categories
Funding
- Postdoctoral Research Foundation of China [20090460415]
- International Science & Technology Cooperation Program of China [2010DFB30550]
- National Natural Science Foundation of China [31101548]
- earmarked fund for China Agriculture Research System [CARS-25-C-13]
Ask authors/readers for more resources
To investigate the response of cucumber seedlings to exogenous salicylic acid (SA) and gain a better understanding of SA action mechanism, we generated a proteomic profile of cucumber (Cucumis sativus L) cotyledons treated with exogenous SA. Analysis of 1500 protein spots from each gel revealed 63 differentially expressed proteins, 59 of which were identified successfully. Of the identified proteins, 97% matched cucumber proteins using a whole cucumber protein database based on the newly completed genome established by our laboratory. The identified proteins were involved in various cellular responses and metabolic processes, including antioxidative reactions, cell defense, photosynthesis, carbohydrate metabolism, respiration and energy homeostasis, protein folding and biosynthesis. The two largest functional categories included proteins involved in antioxidative reactions (23.7%) and photosynthesis (18.6%). Furthermore, the SA-responsive protein interaction network revealed 13 key proteins, suggesting that the expression changes of these proteins could be critical for SA-induced resistance. An analysis of these changes suggested that SA-induced resistance and seedling growth might be regulated in part through pathways involving antioxidative reactions and photosynthesis. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available