Journal
PLANT SCIENCE
Volume 197, Issue -, Pages 50-58Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2012.08.013
Keywords
Capsicum annuum; WRKY; Hypersensitive response; Tobacco mosaic virus; Gene expression
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Funding
- Science Research Center-Engineering Research Center Program (Plant Signaling Network Research Center) of the Ministry of Education, Science and Technology [R11-2003-008-02001-0]
- Mid-career Researcher-Program through a National Research Foundation
- MEST [2009-0085565]
- Wujangchoon Project from the Rural Development Administration, Republic of Korea [11007850]
- National Research Foundation of Korea [2009-0085565] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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WRKY transcription factors regulate biotic, abiotic, and developmental processes. In terms of plant defense, WRKY factors have important roles as positive and negative regulators via transcriptional regulation or protein-protein interaction. Here, we report the characterization of the gene encoding Capsicum annuum WRKY transcription factor d (CaWRKYd) isolated from microarray analysis in the Tobacco mosaic virus (TMV)-P-0-inoculated hot pepper plants. CaWRKYd belongs to the WRKY Ila group, a very small clade in the WRKY subfamily, and WRKY ha group has positive/negative regulatory roles in Arabidopsis and rice. CaWRKYd transcripts were induced by various plant defense-related hormone treatments and TMV-P-0 inoculation. Silencing of CaWRKYd affected TMV-P-0-mediated hypersensitive response (HR) cell death and accumulation of TMV-P-0 coat protein in local and systemic leaves. Furthermore, expression of some pathogenesis-related (PR) genes and HR-related genes was reduced in the CaWRKYd-silenced plants compared with TRV2 vector control plants upon TMV-P-0 inoculation. CaWRKYd was confirmed to bind to the W-box. Thus CaWRKYd is a newly identified Capsicum annuum WRKY transcription factor that appears to be involved in TMV-P-0-mediated HR cell death by regulating downstream gene expression. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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