Journal
PLANT SCIENCE
Volume 181, Issue 5, Pages 509-519Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2011.04.003
Keywords
Nitric oxide measurement; In/Ex planta assays; Griess reagent; Diaminofluorescein (DAF) dyes; NO electrodes; Electron spin resonance; Mass spectrometry; Chemiluminescence; Laser photoacoustic detection; Quantum cascade laser
Categories
Funding
- BBSRC
- EU [FP6-026183]
- Spanish government [AGL2010-15936/AGR]
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Over the last decade nitric oxide (NO) has been shown to influence a range of processes in plants. However, when, where and even if NO production occurs is controversial in several physiological scenarios in plants. This arises from a series of causes: (a) doubts have arisen over the specificity of widely used 4,5-diaminofluorescein diacetate (DAF-2DA)/4-amino-5-methylamino-2,7-difluorofluorescein (OAF-FM) dyes for NO, (b) no plant nitric oxide synthase (NOS) has been cloned, so that the validity of using mammalian NOS inhibitors to demonstrate that NO is being measured is debatable, (c) the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) needs to be used with caution, and (d) some discrepancies between assays for in plan to measurements and another based on sampling NO from the gas phase have been reported. This review will outline some commonly used methods to determine NO, attempt to reconcile differing results obtained by different laboratories and suggest appropriate approaches to unequivocally demonstrate the production of NO. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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