Journal
PLANT SCIENCE
Volume 179, Issue 1-2, Pages 97-102Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2010.04.004
Keywords
Cold tolerance; QTL; F-box protein; Anther length; Rice
Categories
Funding
- Ministry of Agriculture, Forestry, and Fisheries of Japan [QT-3005]
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Low temperatures during the booting stage reduce rice yields by causing cold-induced male sterility. We previously mapped a quantitative trait locus for cold tolerance, Ctb1, to a 56-kb region containing 7 putative genes. In this study we further mapped Ctb1 to a 17-kb region containing two genes that encode an F-box protein and a ser/thr protein kinase. The F-box protein gene was preferentially expressed in young panicles, while the ser/thr protein kinase gene was expressed in leaves and young panicles. Both genes were cloned from a cold-tolerant variety, Norin-PL8, and introduced into a cold-sensitive variety, Hokkai241, and a cold-sensitive line, BT4-74-8. The cold tolerance of T-2 and T-3 progenies was assessed by measuring the degree of spikelet fertility in plants treated with cool water irrigation (19 degrees C, 25cm) or cool air (12 degrees C, 4 days). The results indicated that the F-box protein gene confers cold tolerance. Cold tolerance is associated with greater anther length, and the transgenic plants had longer anthers than non-transformed controls. The F-box protein interacts with a subunit of the E3 ubiquitin ligase, Skp1, suggesting that an ubiquitin-proteasome pathway is involved in cold tolerance at the booting stage. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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