Journal
PLANT SCIENCE
Volume 176, Issue 3, Pages 397-405Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2008.12.007
Keywords
Gossypium; Cotton; EST-SSR; D-genome; Diploid; Tetraploid; Genetic relationship
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Funding
- National Natural Science Foundation of China [30871558, 30730067]
- National High-tech Program [2006AA10Z111]
- Program for New Century Excellent Talents in University [NCET-04-0500]
- Ministry of Education, China [B08025]
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Tetraploid cotton species included two subgenomes, named as A-subgenome and D-subgenome, respectively. Prior to this study, the D-subgenome donor in tetraploid cotton species remained elusive. In a previous study, simple sequence repeats developed from expressed sequence tags (EST-SSRs) of G. raimondii was found to possess a high polymorphic frequency between G. hirsutum and G. barbadense [W.Z. Guo, C.P. Cai, C.B. Wang, Z.G. Han, X.L. Song, K. Wang, X.W. Niu, C. Wang, K.Y. Lu, B. Shi,T.Z. Zhang, A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium, Genetics 176 (2007) 527-541]. We questioned the role of the D-genome cotton species in the evolution of tetraploid cotton. In this paper, 14 EST-SSR primer pairs from G. raimondii were used to amplify 23 species in Gossypium. In total, 438 amplicons were cloned, sequenced and analyzed. A phylogenetic tree of 13 D-genome diploid species and 8 AD-genome tetraploid species was constructed based on the combined simple sequence repeat (SSR) flanking sequence data. The results showed that 13 D-genome species were congruent with Fryxell's subsection taxonomy. The relationship between D-subgenome tetraploid species and D-genome diploid species indicated that G. raimondii is the sole D-genome donor of all tetraploid species. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
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