Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 130, Issue -, Pages 589-603Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2018.08.006
Keywords
Arsenate; Differentially expressed genes; Down regulation; Phosphate; Transcriptomics; Up regulation
Categories
Funding
- National Natural Science Foundation of China [31330055]
- China Agriculture Research System [CARS-05-02A-01]
- Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC- MCP)
Ask authors/readers for more resources
Arsenic (As) is a ubiquitous metalloid and toxic to plants. Chemical similarity between arsenate and phosphate (P) indicates possible antagonism between them in uptake and transportation. However, there is little study to reveal the interaction of As and P at transcriptional level. In this study RNA-sequencing was conducted on the two barley genotypes differing in As tolerance. A total of 2942 differentially expressed genes (DEGs) were inclusively expressed in both genotypes under As (100 mu M) and As (100 mu M) + P (50 mu M), and these DEGs included hormonal signaling, stress responsive, transport related and transcription factors. P addition in the culture solution inhibited the KEGG pathways related to ABC transporters, ether lipid metabolism, linolenic acid metabolism, endocytosis and RNA transport. ZDB160 had a higher expression of DEGs associated with hormone signaling, secondary metabolites and stress defense under P conditions compared to ZDB475, which might explain its tolerance mechanism to As under P condition. The abscisic acid, jasmonic acid and salicylic acid signaling pathways were also significantly regulated under As + P conditions, which may also account for genotypic differences. Finally we drew up a hypothetical model of high As + P stress tolerance mechanism in ZDB160. It may be concluded that ZDB160 achieves its tolerance to As under P by up-regulating P transporters, resulting in more P uptake and less As translocation. The identified candidate genes related to As + P tolerance may provide insights into understanding As tolerance under limited P conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available