Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 75, Issue -, Pages 9-13Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2013.11.027
Keywords
Chloroplast; Glutathione; Immunolocalization; Stroma; Arabidopsis
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Funding
- National Science Foundation [MCB-0904215]
- NSF
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In plants, glutathione serves as a versatile redox buffer and cellular protective compound against a range of biotic and abiotic stresses. Glutathione production involves glutamate-cysteine ligase (GCL), the redox-regulated limiting enzyme of the pathway, and glutathione synthetase (GS). Because the sub-cellular and sub-organellar localization of these enzymes will have an impact on metabolism, here we examine the localization of GCL and GS in the leaves of Arabidopsis thaliana. Immuno-electron microscopy of leaf cells indicates localization of GCL primarily to the chloroplast with GS found in both the chloroplast and cytosol. Detailed examination of the localization of both enzymes within chloroplasts was performed using fractionation followed by immunoblot analysis and indicates that GCL and GS are found in the stroma. The localization of these enzymes to the stroma of chloroplasts has implications for the redoxregulation of GCL and plant glutathione biosynthesis. (C) 2013 Elsevier Masson SAS. All rights reserved.
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