Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 63, Issue -, Pages 82-88Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2012.11.019
Keywords
Chlorogenic acid; Flavonols; Free amino acids; Lignin; Phenolic metabolism
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Funding
- European Regional Development Fund [CZ.1.05/1.1.00/02.0068]
- OP Education for Competitiveness [CZ.1.07/2.3.00/30.0017]
- grant agency of the Czech Republic [GA CR 525/07/0338]
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Accumulation of secondary metabolites (general phenols, naphthodianthrones and phloroglucinol hyperforin) in Hypericum perforatum and Hypericum canariense after application of the inhibitor (2-aminoindane-2-phosphonic acid, AIP) of the pivotal enzyme of general phenylpropanoid pathway (phenylalanine ammonia-lyase, PAL) was studied. Shoots of H. perforatum revealed more expressive growth depression, concomitantly with the inhibition of PAL activity (-60%) and decrease in soluble phenols and individual phenolic acids in response to AIP. Hypericins (hypericin, pseudohypericin and protohypericin) decreased while hyperforin increased in AIP-cultured H. perforatum. On the contrary, growth changes, decreases in soluble phenols and individual phenolic acids were less-visible in H. canariense. This was also reflected in restoration of PAL activity (+330%) and selected flavonoids even increased. Hypericins and hyperforin were present in several orders of magnitude lower amounts in comparison with H. perforatum. Increase in proline indicates potential compensatory antioxidative mechanism if phenols are depleted. Microscopy revealed also differences in secondary xylem formation and lignification between species after exposure to AIP. (c) 2012 Elsevier Masson SAS. All rights reserved.
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