Effect of sulfur dioxide on ROS production, gene expression and antioxidant enzyme activity in Arabidopsis plants

Sulfur dioxide (SO2) is one of the most common and harmful air pollutants. To analyze antioxidant response of plants to SO2 stress, we investigated the reactive oxygen species (ROS) levels, transcript alterations and antioxidant enzyme activities in Arabidopsis thaliana (Col-0) exposed to 0, 2.5, 10 and 30 mg m(-3) of SO2. The results showed that both superoxide radical (O-2(-center dot)) generation rate and hydrogen peroxide (H2O2) content increased in SO2-treated Arabidopsis shoots. GeneChip and RT-PCR analysis revealed that transcript levels of peroxidase (POD), glutathione peroxidase (GPX) and superoxide dismutase (SOD) genes enhanced after exposure to 30 mg m(-3) SO2 for 72 h. The content of glutathione and activities of SOD, POD and GPX increased significantly during 72 h of SO2 exposure. However, catalases (CAT) activity changed slightly under SO2 stress. Furthermore, the results of in-gel enzyme assays indicated that SOD (FeSOD and CugnSOD) and POD isoforms increased after exposure to SO2 for 72 h, whereas two CAT isoforms (CAT2 and CAT3) declined. Malondialdehyde content kept at a low level within 72 h of SO2 exposure, but increased significantly after exposure to 30 mg m(-3) SO2 for 120 h along with decrease in the level of ROS and activities of SOD and GPX. Our results indicated that increased ROS may act as a signal to induce defense response to SO2 stress. Antioxidant status plays an important role in plant protection against SO2-caused oxidative stress, though the defense capacity cannot sufficiently alleviate oxidative damage occurring under prolonged exposure to higher concentrations of SO2. (C) 2012 Elsevier Masson SAS. All rights reserved.