Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 49, Issue 10, Pages 1100-1107Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2011.07.005
Keywords
Plant defensin; Antimicrobial peptide; Syncytium; Heterodera schachtii; Arabidopsis; Roots
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Funding
- Austrian Science Fund (FWF) [P16296-B06, P21067-B12, P20471-B11]
- Higher Education Commission (HEC) of Pakistan
- Baxter AG
- Austrian Research Centres (ARC) Seibersdorf
- Austrian Centre of Biopharmaceutical Technology (ACBT)
- Vienna Science and Technology Fund (WWTF)
- Austrian Science Fund (FWF) [P20471, P21067] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [P 20471, P 21067] Funding Source: researchfish
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The beet cyst nematode Heterodera schachtii induces a feeding site, called syncytium, in roots of host plants. In Arabidopsis, one of the genes whose expression is strongly induced in these structures is Pdf2.1 which codes for an antimicrobial plant defensin. Arabidopsis has 13 plant defensin genes. Besides Pdf2.1, the Pdj2.2 and Pdf2.3 genes were strongly expressed in syncytia and therefore the expression of all three Pdf genes was studied in detail. The promoter of the Pdj2.1 gene turned out to be an interesting candidate to drive a syncytium-specific expression of foreign genes as RT-PCR showed that apart from the feeding site it was only expressed in siliques (seeds). The Pdj2.2 and Pdj2.3 genes were in addition expressed in seedlings, roots, leaves, stems, and flowers. These results were supported by the analysis of promoter::GUS lines. After infection with H. schachtii all GUS lines showed a strong staining in syncytia at 5 and 15 dpi. This expression pattern was confirmed by in situ RT-PCR. (C) 2011 Elsevier Masson SAS. All rights reserved.
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