Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 49, Issue 2, Pages 230-234Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2010.11.012
Keywords
Lipoxygenase; Oil bodies; Oilseed; Seed germination
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Funding
- Alexander von Humboldt Foundation (Germany)
- C.S.I.R, New Delhi
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Lipoxygenase (linoleate:oxygen oxidoreductase: EC 1.13.11.12: LOX) catalyzes oxygenation of polyenoic fatty acids, which precedes the degradation of storage lipids during seed germination in sunflower. In the present work, it has been confirmed that 2',7'-dichlorodihydrofluorescein diacetate (H(2)DCFDA) produces fluorescence in presence of lipid hydroperoxides (LOX reaction products). This work provides new information on spatial localization of transiently enhanced LOX activity in protoplasts from 5 d old seedling cotyledons of sunflower (Helianthus annuus L cv. Morden) by exploiting H(2)DCFDA as a probe for fluorescence detection from LOX activity sites. Use of LOX inhibitors [nordihydroguaiaretic acid (NDGA) and propyl gallate (PG)] confirms oil bodies as LOX activity sites. Oil body surface has been shown to possess LOX activity in 5 d old seedling cotyledons. (C) 2010 Elsevier Masson SAS. All rights reserved.
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