Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 46, Issue 4, Pages 421-427Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2007.09.013
Keywords
catalase; crassulacean acid metabolism; molecular mass; Mesembryanthemum crystallinum; salinity; subunits
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Catalase (CAT; EC 1.11.1.6) isolated from leaves of the halophytic plant Mesembryanthemum crystallinum is characterized by a high apparent molecular mass of about 320 kDa, and high resistance to denaturing agents (10% ME). SIDS-treatment breaks active oligomeric CAT into the less active and putatively dimeric form of 160 kDa apparent molecular mass. Three subunits are resolved after denaturing PAGE: 79, 74 and 62 kDa. Higher molecular masses of subunits coincide with increased activity of CAT. M. crystallinum leaf CAT reveals a diel variation in the resistance to denaturing factors and the stability of CAT is increased in a light-dependent manner both in C-3- and in CAM-induced plants. Unchanged level of leaf CAT transcripts is documented in the diurnal cycle of C-3 plants and after salinity-induced crassulacean acid metabolism (CAM). (C) 2007 Elsevier Masson SAS. All rights reserved.
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