Journal
PLANT PHYSIOLOGY
Volume 178, Issue 3, Pages 1310-1331Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.18.00603
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Funding
- EU
- Dutch Top Technology Institute Green Genetics
- Dutch Technology Foundation STW
- Earth and Life Sciences ALW, which are part of the Netherlands Organization for Scientific Research (NWO)
- UEFISCDI [PN-III-ID-PCE-2016-0650]
- IBAR from the Romanian Academy [1, 3]
- Biotechnology and Biological Sciences Research Council [BB/M007405/1]
- COST Action [SUSTAIN FA1208]
- BBSRC [BB/M007405/1] Funding Source: UKRI
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The intracellular immune receptor Rx1 of potato (Solanum tuberosum), which confers effector-triggered immunity to Potato virus X, consists of a central nucleotide-binding domain (NB-ARC) flanked by a carboxyl-terminal leucine-rich repeat (LRR) domain and an amino-terminal coiled-coil (CC) domain. Rxl activity is strictly regulated by interdomain interactions between the NB-ARC and LRR, but the contribution of the CC domain in regulating Rxl activity or immune signaling is not fully understood. Therefore, we used a structure-informed approach to investigate the role of the CC domain in Rxl functionality. Targeted mutagenesis of CC surface residues revealed separate regions required for the intramolecular and intermolecular interaction of the CC with the NB-ARC-LRR and the cofactor Ran GTPase-activating protein2 (RanGAP2), respectively. None of the mutant Rxl proteins was constitutively active, indicating that the CC does not contribute to the autoinhibition of Rxl activity. instead, the CC domain acted as a modulator of downstream responses involved in effector-triggered immunity. Systematic disruption of the hydrophobic interface between the four helices of the CC enabled the uncoupling of cell death and disease resistance responses. Moreover, a strong dominant negative effect on Rxl-mediated resistance and cell death was observed upon coexpression of the CC alone with full-length Rxl protein, which depended on the RanGAP2-binding surface of the CC. Surprisingly, coexpression of the N-terminal half of the CC enhanced Rxl-mediated resistance, which further indicated that the CC functions as a scaffold for downstream components involved in the modulation of disease resistance or cell death signaling.
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