Journal
PLANT PHYSIOLOGY
Volume 166, Issue 2, Pages 455-U42Publisher
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.114.239392
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Funding
- National Science Foundation (Plant Genome) [1238243, 1052395]
- Finnish Cultural Foundation postdoctoral fellowship
- Engineering and Physical Sciences Research Council
- National Science Foundation [EP/H019162/1]
- Comision Nacional de Investigacion Cientifica y Tecnologica Programa de Atraccion e Insercion de Capital Humano Avanzado [82130027]
- Direct For Biological Sciences [1052395] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1238243] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems [1052395] Funding Source: National Science Foundation
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Agrobacterium rhizogenes (or Rhizobium rhizogenes) is able to transform plant genomes and induce the production of hairy roots. We describe the use of A. rhizogenes in tomato (Solanum spp.) to rapidly assess gene expression and function. Gene expression of reporters is indistinguishable in plants transformed by Agrobacterium tumefaciens as compared with A. rhizogenes. A root cell type- and tissue-specific promoter resource has been generated for domesticated and wild tomato (Solanum lycopersicum and Solanum pennellii, respectively) using these approaches. Imaging of tomato roots using A. rhizogenes coupled with laser scanning confocal microscopy is facilitated by the use of a membrane-tagged protein fused to a red fluorescent protein marker present in binary vectors. Tomato-optimized isolation of nuclei tagged in specific cell types and translating ribosome affinity purification binary vectors were generated and used to monitor associated messenger RNA abundance or chromatin modification. Finally, transcriptional reporters, translational reporters, and clustered regularly interspaced short palindromic repeats-associated nuclease9 genome editing demonstrate that SHORT-ROOT and SCARECROW gene function is conserved between Arabidopsis (Arabidopsis thaliana) and tomato.
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