Journal
PLANT PHYSIOLOGY
Volume 166, Issue 4, Pages 1803-1820Publisher
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.114.248716
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Funding
- National Science Foundation [DBI 0820580, MCB 1330337]
- Hellman Fellows Fund
- U.S. Department of Agriculture National Institute of Food and Agriculture (Hatch) [CA-D-PLS-7033-H]
- Danish National Research Foundation [DNRF99]
- Direct For Biological Sciences [1021861] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems [1021861] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1330337] Funding Source: National Science Foundation
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A key unanswered question in plant biology is how a plant regulates metabolism to maximize performance across an array of biotic and abiotic environmental stresses. In this study, we addressed the potential breadth of transcriptional regulation that can alter accumulation of the defensive glucosinolate metabolites in Arabidopsis (Arabidopsis thaliana). A systematic yeast one-hybrid study was used to identify hundreds of unique potential regulatory interactions with a nearly complete complement of 21 promoters for the aliphatic glucosinolate pathway. Conducting high-throughput phenotypic validation, we showed that >75% of tested transcription factor (TF) mutants significantly altered the accumulation of the defensive glucosinolates. These glucosinolate phenotypes were conditional upon the environment and tissue type, suggesting that these TFs may allow the plant to tune its defenses to the local environment. Furthermore, the pattern of TF/promoter interactions could partially explain mutant phenotypes. This work shows that defense chemistry within Arabidopsis has a highly intricate transcriptional regulatory system that may allow for the optimization of defense metabolite accumulation across a broad array of environments.
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