Journal
PLANT PHYSIOLOGY
Volume 163, Issue 3, Pages 1230-1241Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.113.226050
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Funding
- Ministero dell'Istruzione, dell'Universita e della Ricerca Fondo per gli Investimenti della Ricerca di Base [2010 RBFR10S1LJ_001]
- German Research Foundation [FOR 964, SFB 629]
- International Max Planck Research School-Cell Dynamics and Disease
- binational Deutscher Akademischer Austausch Dienst/VIGONI grant
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In planta, very limited information is available about how the endoplasmic reticulum (ER) contributes to cellular Ca2+ dynamics and homeostasis. Here, we report the generation of an ER-targeted Cameleon reporter protein suitable for analysis of Ca2+ accumulation and dynamics in the lumen of the ER in plant cells. Using stably transformed Arabidopsis (Arabidopsis thaliana) plants expressing this reporter protein, we observed a transiently enhanced accumulation of Ca2+ in the ER in response to stimuli inducing cytosolic Ca2+ rises in root tip cells. In all experimental conditions, ER Ca2+ dynamics were substantially different from those monitored in the cytosol. A pharmacological approach enabled us to evaluate the contribution of the different ER-resident Ca2+-ATPase classes in the regulation of the ER Ca2+ homeostasis. Taken together, our results do not provide evidence for a role of the ER as a major source that releases Ca2+ for stimulus-induced increases in cytosolic Ca2+ concentration. Instead, our results show that the luminal ER Ca2+ elevations typically follow cytosolic ones, but with distinct dynamics. These findings suggest fundamental differences for the function of the ER in cellular Ca2+ homeostasis in plants and animals.
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