Journal
PLANT PHYSIOLOGY
Volume 162, Issue 2, Pages 707-719Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.113.216218
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- Deutsche Forschungsgemeinschaft
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This study dealt with the visualization of the sieve element (SE) cytoskeleton and its involvement in electrical responses to local cold shocks, exemplifying the role of the cytoskeleton in Ca2+-triggered signal cascades in SEs. High-affinity fluorescent phalloidin as well as immunocytochemistry using anti-actin antibodies demonstrated a fully developed parietal actin meshwork in SEs. The involvement of the cytoskeleton in electrical responses and forisome conformation changes as indicators of Ca2+ influx was investigated by the application of cold shocks in the presence of diverse actin disruptors (latrunculin A and cytochalasin D). Under control conditions, cold shocks elicited a graded initial voltage transient, Delta V-1, reduced by external La3+ in keeping with the involvement of Ca2+ channels, and a second voltage transient, Delta V-2. Cytochalasin D had no effect on Delta V-1, while Delta V-1 was significantly reduced with 500 nM latrunculin A. Forisome dispersion was triggered by cold shocks of 4 degrees C or greater, which was indicative of an all-or-none behavior. Forisome dispersion was suppressed by incubation with latrunculin A. In conclusion, the cytoskeleton controls cold shock-induced Ca2+ influx into SEs, leading to forisome dispersion and sieve plate occlusion in fava bean (Vicia faba).
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