Journal
PLANT PHYSIOLOGY
Volume 149, Issue 1, Pages 384-394Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.108.128066
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Funding
- National Science Foundation [MCB-0718152, MCB-0718064]
- Japan Society for the Promotion of Science Postdoctoral Fellowship
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The phenylpropene t-anethole imparts the characteristic sweet aroma of anise (Pimpinella anisum, family Apiaceae) seeds and leaves. Here we report that the aerial parts of the anise plant accumulate t-anethole as the plant matures, with the highest levels of t-anethole found in fruits. Although the anise plant is covered with trichomes, t-anethole accumulates inside the leaves and not in the trichomes or the epidermal cell layer. We have obtained anise cDNA encoding t-anol/isoeugenol synthase 1 (AIS1), an NADPH-dependent enzyme that can biosynthesize t-anol and isoeugenol (the latter not found in anise) from coumaryl acetate and coniferyl acetate, respectively. In addition, we have obtained a cDNA encoding S-[methyl-C-14] adenosyl-L-methionine: t-anol/isoeugenol O-methyltransferase 1 (AIMT1), an enzyme that can convert t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group. The genes encoding AIS1 and AIMT1 were expressed throughout the plant and their transcript levels were highest in developing fruits. The AIS1 protein is 59% identical to petunia (Petunia hybrida) isoeugenol synthase 1 and displays apparent K-m values of 145 mu M for coumaryl acetate and 230 mu M for coniferyl acetate. AIMT1 prefers isoeugenol to t-anol by a factor of 2, with K-m values of 19.3 mu M for isoeugenol and 54.5 mu M for S-[methyl-C-14] adenosyl-L-methionine. The AIMT1 protein sequence is approximately 40% identical to basil (Ocimum basilicum) and Clarkia breweri phenylpropene O-methyltransferases, but unlike these enzymes, which do not show large discrimination between substrates with isomeric propenyl side chains, AIMT1 shows a 10-fold preference for t-anol over chavicol and for isoeugenol over eugenol.
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