Journal
PLANT PHYSIOLOGY
Volume 147, Issue 1, Pages 30-40Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.107.115246
Keywords
-
Categories
Ask authors/readers for more resources
We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available