Journal
PLANT PATHOLOGY JOURNAL
Volume 25, Issue 1, Pages 108-111Publisher
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.2009.25.1.108
Keywords
fungi; genomic DNA extraction; high-throughput
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Funding
- Rural Development Administration [20080401-034-044-008-01-00]
- Ministry of Science and Technology [CG1141]
- Korea government (MEST) [R11-2008-062-03001-0]
- Ministry of Education through the Brain Korea 21 Program
- National Research Foundation of Korea [R11-2008-062-03001-0, 과C6A2206] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [PJ00700820091136300] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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DNA-based studies, including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating phenol-chloroform extraction procedures, and by deploying a simple electric grinder. This QS method is applicable not only to a broad range of microbial eukaryotes, including true fungi and oomycetes, but also to lichens and plants.
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