Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 30, Issue 1, Pages 187-194Publisher
SPRINGER
DOI: 10.1007/s11105-011-0321-6
Keywords
Rice (Oryza sativa L.); SDG gene GA-insensitive mutant; Semidwarf; Phenotypic complementation
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Funding
- National Natural Science Foundation of PR China [31071068]
- Bureau of Education, Jiangsu Province, PR China [10KJA210059]
- Ministry of Science and Technology of PR China [2009ZX08009-100B]
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A semidwarf line of Indica rice, Xinguiai, was derived from the progeny of a cross between the double dwarf mutant Xinguiaishuangai and the wild-type variety Nanjing 6. The semidwarf phenotype was controlled by the semidwarf gene, sdg. The second sheath and shoot elongation responses of the dwarf mutant to exogenous gibberellin (GA(3)) showed that sdg was insensitive to gibberellin (GA), and its endogenous GAs content was higher than that in wild-type cultivars. The SDG gene was cloned by a map-based cloning method and sequencing analysis revealed that the coding region of sdg had a single nucleotide substitution resulting in a single amino acid change from alanine to threonine. A cleaved amplified polymorphic sequence marker was designed according to sequences from mutant and wild-type materials. This sequence marker could be used to distinguish wild types and mutants, and thus, could be used for molecular marker-assisted selection. The dwarf phenotype of the sdg mutant was restored to a normal phenotype by introducing the wild-type SDG gene. Rice transformation experiments and GUS staining demonstrated that the SDG gene was predominantly expressed in vegetative organs.
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