Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 27, Issue 3, Pages 305-314Publisher
SPRINGER
DOI: 10.1007/s11105-008-0086-8
Keywords
Tall fescue (Festuca arundinacea); Class I chitinase; Promoter activation; Stress-induced gene; Plant defense
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Funding
- National Natural Science Foundation of China [30870194]
- Natural Science Foundation of Shaanxi Province [2006C103]
- Research Project of Provincial Key Laboratory of Shaanxi [04JS07, 08JZ70]
- Scientific Research Project of the Education Department of Shaanxi Province [05JK304, 08JK466]
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The cDNA, genomic DNA, and promoter sequence of FaChit1, a class I chitinase gene from Festuca arundinacea, were isolated and characterized in the present work. The deduced amino acid sequence of FaChit1 contains the chitin binding, catalytic, and proline and glycine-rich domains characteristic for most class I chitinases, but no C-terminal extension region. FaChit1 is induced effectively by fungal elicitors, dehydration, and ethylene, but only slightly by mechanical wounding. To identify potential stress-related cis-acting elements, 5' sequences 935, 651, and 233 bp upstream of the FaChit1 start codon were fused to the GUS reporter gene and analyzed in transgenic tobacco. The results indicated that the 935 bp fragment closely mirrored endogenous gene expression and that the 651 bp fragment was sufficient to direct reporter the gene expression in response to fungal elicitors, ethylene, dehydration, or mechanical wounding due to both known and presently uncharacterized cis-acting elements.
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