Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 27, Issue 3, Pages 350-354Publisher
SPRINGER
DOI: 10.1007/s11105-009-0092-5
Keywords
B chromosome; DOP-PCR; Evolution; Microdissection; Zea mays
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Funding
- Ministry of Education, Taiwan, R. O. C.
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Understanding the evolution of the maize B chromosome requires insight into the molecular organization of a large number of B clones, which can be potentially obtained by microdissection of the chromosome. Yet, the microdissection protocols currently available are ineffective for a large-scale isolation. In an attempt to improve its efficiency, a protocol was adopted to screen a microdissected B library with probes prepared from the degenerate oligonucleotide primed-PCR product of genomic DNA. This protocol resulted in 59 new B clones, most of which were highly repetitive sequences located in various B regions but mostly in the heterochromatic blocks of the long arm. They also appeared in A chromosomes. Twenty-four of these were retrotransposons, ten knob, 18 noncoding sequences, and seven unknown sequences. The implication of the new B sequences on the B evolution is discussed.
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