Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 26, Issue 3, Pages 186-198Publisher
SPRINGER
DOI: 10.1007/s11105-008-0029-4
Keywords
DNA isolation; Glass fiber plates; Plants; Automation; DNA barcoding
Categories
Funding
- Genome Canada
- Canada Foundation for Innovation
- Ontario Innovation Trust
- Canada Research Chairs Program
- NSERC
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Many plant species are considered difficult for DNA isolation due to their high concentrations of secondary metabolites such as polysaccharides and polyphenols. Several protocols have been developed to overcome this problem, but they are typically time-consuming, not scalable for high throughput and not compatible with automation. Although a variety of commercial kits are available for plant DNA isolation, their cost is high and these kits usually have limited taxonomic applicability. In a previous study we developed an inexpensive automation-friendly protocol for DNA extraction from animal tissues. Here we demonstrate that a similar protocol allows DNA isolation from plants.
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