Journal
PLANT MOLECULAR BIOLOGY
Volume 73, Issue 4-5, Pages 363-378Publisher
SPRINGER
DOI: 10.1007/s11103-010-9628-1
Keywords
Promoter; Transgene; Multigene transformation; Transcriptional silencing; Constitutive; Spatiotemporal; Inducible
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Funding
- Ministry of Science and Innovation, Spain [BFU2007-61413, BIO2007-30738-E]
- European Research Council Advanced Grant (BIOFORCE)
- MICINN, Spain
- ICREA Funding Source: Custom
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Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.
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