Journal
PLANT MOLECULAR BIOLOGY
Volume 68, Issue 3, Pages 239-250Publisher
SPRINGER
DOI: 10.1007/s11103-008-9365-x
Keywords
Oryza Sativa L; rolled leaf; KANADI; RL9
Categories
Funding
- Ministry of Science and Technology of China [2005CB120804]
- Jiangsu Innovation Talent Project [BK2006506]
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Leaves, the collective organ produced by the shoot apical meristem (SAM), are polarized along their adaxial-abaxial axis. In this study, we characterized two rice (Oryza sativa) allelic rolled-leaf mutants, rolled leaf 9-1 (rl9-1) and rl9-2, which display very similar phenotypes with completely adaxialized leaves and malformed spikelets. We cloned the RL9 gene by way of a map-based cloning strategy. Molecular studies have revealed that RL9 encodes a GARP protein, an orthologue of Arabidopsis KANADIs. RL9 is mainly expressed in roots, leaves, and flowers. The transient expression of a RL9-GFP (green fluorescent protein) fusion protein has indicated that RL9 protein is localized in the nucleus, suggesting that RL9 acts as a putative transcription factor.
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