Journal
PLANT METHODS
Volume 9, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1746-4811-9-1
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Funding
- Fonds pour la formation a la Recherche dans l'Industrie et dans l'Agriculture
- Belgian Science Policy Office [IAP7/29]
- Communaute francaise de Belgique [ARC-1116-036]
- European Community [FP7-244374]
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This paper presents an original spit-and-combine imaging procedure that enables the complete vectorization of complex root systems grown in rhizotrons. The general principle of the method is to (1) separate the root system into a small number of large pieces to reduce root overlap, (2) scan these pieces one by one, (3) analyze separate images with a root tracing software and (4) combine all tracings into a single vectorized root system. This method generates a rich dataset containing morphological, topological and geometrical information of entire root systems grown in rhizotrons. The utility of the method is illustrated with a detailed architectural analysis of a 20-day old maize root system, coupled with a spatial analysis of water uptake patterns.
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