Journal
PLANT METHODS
Volume 7, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1746-4811-7-47
Keywords
chloroplast; plastid transcription; promoters; RNA processing; run on transcription assay; RNase protection assay
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Funding
- Deutsche Forschungsgemeinschaft [SFB 429]
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Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.
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