Journal
PLANT METHODS
Volume 7, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1746-4811-7-46
Keywords
Composite plants; gene complementation; mycorrhizae; nodulation; pea mutants; targeted spot-inoculation; transformation
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Funding
- Natural Sciences and Engineering Research Council of Canada
- Wilfrid Laurier University
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Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses.
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