Journal
PLANT JOURNAL
Volume 73, Issue 2, Pages 325-335Publisher
WILEY
DOI: 10.1111/tpj.12043
Keywords
darkness; Starvation; calmodulin-like; environmental stress; calcium signaling; abiotic stress; macroautophagy; protein-protein interaction
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Funding
- US National Science Foundation [MCB 0817976]
- Virginia and L.E. Simmons Family Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0817976] Funding Source: National Science Foundation
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Plants encounter environmental stress challenges that are distinct from those of other eukaryotes because of their relative immobility. Therefore, plants may have evolved distinct regulatory mechanisms for conserved cellular functions. Plants, like other eukaryotes, share aspects of both calcium-and calmodulin-based cellular signaling and the autophagic process of cellular renewal. Here, we report a novel function for an Arabidopsis calmodulin-related protein, CML24, and insight into ATG4-regulated autophagy. CML24 interacts with ATG4b in yeast two-hybrid, in vitro pull-down and transient tobacco cell transformation assays. Mutants with missense mutations in CML24 have aberrant ATG4 activity patterns in in vitro extract assays, altered ATG8 accumulation levels, an altered pattern of GFP-ATG8-decorated cellular structures, and altered recovery from darkness-induced starvation. Together, these results support the conclusion that CML24 affects autophagy progression through interactions with ATG4.
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