Journal
PLANT JOURNAL
Volume 66, Issue 3, Pages 492-501Publisher
WILEY
DOI: 10.1111/j.1365-313X.2011.04507.x
Keywords
cap-independent translational enhancer; Melon necrotic spot virus; Nicotiana benthamiana; non-host resistance; translation initiation factor eIF4E; susceptibility factor
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Funding
- Ministerio de Ciencia e Innovacion, Spain [AGL2009-07552/AGR, EUI2009-04009]
- Fundacion Seneca (Region de Murcia, Spain) [05702/PI/07]
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Nicotiana benthamiana has been described as non-host for Melon necrotic spot virus (MNSV). We investigated the basis of this resistance using the unique opportunity provided by strain MNSV-264, a recombinant virus that is able to overcome the resistance. Analysis of chimeric MNSV mutants showed that virulence in N. benthamiana is conferred by a 49 nucleotide section of the MNSV-264 3'-UTR, which acts in this host as a cap-independent translational enhancer (3'-CITE). Although the 3'-CITE of non-adapted MNSV-M alpha 5 is active in susceptible melon, it does not promote efficient translation in N. benthamiana, thus preventing expression of proteins required for virus replication. However, MNSV-M alpha 5 gains the ability to multiply in N. benthamiana cells if eIF4E from a susceptible melon variety (Cm-eIF4E-S) is supplied in trans. These data show that N. benthamiana resistance to MNSV-M alpha 5 results from incompatibility between the MNSV-M alpha 5 3'-CITE and N. benthamiana eIF4E in initiating efficient translation of the viral genome. Therefore, non-host resistance conferred by the inability of a host susceptibility factor to support viral multiplication may be a possible mechanism for this type of resistance to viruses.
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