Journal
PLANT JOURNAL
Volume 68, Issue 1, Pages 64-73Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-313X.2011.04665.x
Keywords
vacuolar membrane; bulbs; SNARE; phospholipase; sgr mutants; Arabidopsis
Categories
Funding
- Ministry of Education, Science, Sports and Culture of Japan
- Science and Technology Agency of Japan
- RIKEN
- Grants-in-Aid for Scientific Research [20001009, 11J08803, 21370016, 23012010] Funding Source: KAKEN
Ask authors/readers for more resources
The plant vacuole fulfills a variety of functions, and is essential for plant growth and development. We previously identified complex and mobile structures on the continuous vacuolar membrane, which we refer to as 'bulbs'. To ascertain their biological significance and function, we searched for markers associated with bulbs, and mutants that show abnormalities with respect to bulbs. We observed bulb-like structures after expression of non-membranous proteins as well as the functional soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) molecules VAM3 and VTI11. Bulbs are formed in more tissues than previously reported, including flowering organs, suspension culture cells, endodermal cells in the flowering stem, and at very early stages of seed germination. Using existing and newly developed marker lines, we found that the frequency of bulb occurrence is significantly decreased in multiple shoot gravitropism (sgr) mutants, which are known to have a defect in vacuolar membrane properties in endodermal cells. Based on results with new marker lines, which enabled us to observe the process of bulb biogenesis, and analysis of the phenotypes of these mutants, we propose multiple mechanisms for bulb formation, one of which may be that used for formation of transvacuolar strands.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available