Journal
PLANT JOURNAL
Volume 63, Issue 1, Pages 141-154Publisher
WILEY
DOI: 10.1111/j.1365-313X.2010.04228.x
Keywords
arbuscular mycorrhizal symbiosis; CCaMK; common symbiosis genes; intracellular infection; root nodule symbiosis
Categories
Funding
- Program of Basic Research Activities for Innovative Biosciences
- Ministry of Agriculture, Forestry and Fisheries of Japan [PMI-0001]
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P>In legumes, Ca2+/calmodulin-dependent protein kinase (CCaMK) is a component of the common symbiosis genes that are required for both root nodule (RN) and arbuscular mycorrhiza (AM) symbioses and is thought to be a decoder of Ca2+ spiking, one of the earliest cellular responses to microbial signals. A gain-of-function mutation of CCaMK has been shown to induce spontaneous nodulation without rhizobia, but the significance of CCaMK activation in bacterial and/or fungal infection processes is not fully understood. Here we show that a gain-of-function CCaMKT265D suppresses loss-of-function mutations of common symbiosis genes required for the generation of Ca2+ spiking, not only for nodule organogenesis but also for successful infection of rhizobia and AM fungi, demonstrating that the common symbiosis genes upstream of Ca2+ spiking are required solely to activate CCaMK. In RN symbiosis, however, CCaMKT265D induced nodule organogenesis, but not rhizobial infection, on Nod factor receptor (NFRs) mutants. We propose a model of symbiotic signaling in host legume plants, in which CCaMK plays a key role in the coordinated induction of infection thread formation and nodule organogenesis.
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