Journal
PLANT JOURNAL
Volume 62, Issue 5, Pages 829-839Publisher
WILEY
DOI: 10.1111/j.1365-313X.2010.04195.x
Keywords
tobacco mosaic virus; microtubules; movement protein; Nicotiana tabacum SR1:nn cv; 'Petit Havana'; activation tagging
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Funding
- German-French Procope Programme
- State of Baden-Wurttemberg (Landesgraduiertenforderung)
- Generalidad Valenciana, Spain [CTBPDC/2204/015, BPOSTDOC06/072]
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P>A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2, alpha-tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus-end marker GFP-EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell-to-cell movement of TMV encoding GFP-tagged movement protein (MP-GFP) is reduced in ATER2 accompanied by a reduced association of MP-GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.
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