Journal
PLANT JOURNAL
Volume 60, Issue 3, Pages 539-550Publisher
WILEY
DOI: 10.1111/j.1365-313X.2009.03978.x
Keywords
Arabidopsis; 14-3-3; RPW8; 2; powdery mildew; resistance; SA signaling
Categories
Funding
- National Research Initiative of the US Department of Agriculture Cooperative State Research, Education and Extension Service [2006-3501-16883]
- Biotechnology and Biological Sciences Research Council [83/P15679]
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P>The RPW8 locus from Arabidopsis thaliana Ms-0 includes two functional paralogous genes (RPW8.1 and RPW8.2) and confers broad-spectrum resistance via the salicylic acid-dependent signaling pathway to the biotrophic fungal pathogens Golovinomyces spp. that cause powdery mildew diseases on multiple plant species. To identify proteins involved in regulation of the RPW8 protein function, a yeast two-hybrid screen was performed using RPW8.2 as bait. The 14-3-3 isoform lambda (designated GF14 lambda) was identified as a potential RPW8.2 interactor. The RPW8.2-GF14 lambda interaction was specific and engaged the C-terminal domain of RPW8.2, which was confirmed by pulldown assays. The physiological impact of the interaction was revealed by knocking down GF14 lambda by T-DNA insertion, which compromised basal and RPW8-mediated resistance to powdery mildew. In addition, over-expression of GF14 lambda resulted in hypersensitive response-like cell death and enhanced resistance to powdery mildew via the salicylic acid-dependent signaling pathway. The results from this study suggest that GF14 lambda may positively regulate the RPW8.2 resistance function and play a role in enhancing basal resistance in Arabidopsis.
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