Journal
PLANT FOODS FOR HUMAN NUTRITION
Volume 67, Issue 4, Pages 365-370Publisher
SPRINGER
DOI: 10.1007/s11130-012-0320-2
Keywords
Opuntia joconostle seeds; Hypolipidemic activity; Phenolic compounds; Hypercholesterolemia
Funding
- Consejo Nacional de Ciencia y Tecnologia (CONACyT) [174690]
- Secretaria de Investigacion y Posgrado-IPN Proyect [20100788]
- Comision de Operacion y Fomento de Actividades Academicas del IPN (COFAA-IPN)
- Universidad Autonoma del Estado de Mexico
- Ohio State University, Food Industries Center
Ask authors/readers for more resources
The purpose of this study was to evaluate the hypolipidemic effect of a methanolic extract from Opuntia joconostle seeds fed to mice in a hypercholesterolemic diet. Acute toxicity of the methanolic extract was investigated by an established method. Phenolic composition and antioxidant activity were determined by high-performance liquid chromatography and DPPH, respectively. The total phenolic content of Opuntia joconostle seeds was 47.85 +/- 1.29 mg gallic acid equivalents/g dry weight. The main phenolic compounds were identified as quercetin, rutin, and cafeic acid. Percent inhibition of DPPH+ was 49.76 +/- 0.49 %. The oral LD50 for the methanolic extract from the Opuntia joconostle seeds was > 5,000 mg/kg BW. Mice fed a hypercholesterolemic diet for six days exhibited significantly (P a parts per thousand currency signaEuro parts per thousand 0.001) higher plasma lipid levels than mice fed a normal diet. Remarkably, supplementation with methanolic extract from Opuntia joconostle at doses of 1, 2, and 5 g/kg body weight significantly (P a parts per thousand currency signaEuro parts per thousand 0.001) prevented the increase in total cholesterol, low-density lipoprotein cholesterol, triglycerides level, and atherogenic index. Similar concentrations of the HDL cholesterol were observed in both treated and control groups. A significant dose-dependent reduction in lipid levels was noted for treated groups compared to the hypercholesterolemic group. We attribute this result to the seeds' phenolic composition. This methanolic extract has potential to be included in short-term hypercholesterolemia treatment regimens as it exhibits hypolipidemic activity with no apparent toxic manifestations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available