Journal
PLANT FOODS FOR HUMAN NUTRITION
Volume 66, Issue 3, Pages 270-274Publisher
SPRINGER
DOI: 10.1007/s11130-011-0245-1
Keywords
Flaxseed; Phenolic compounds; Lignan macromolecule; Caffeic acid
Funding
- Polish Ministry of Science and Higher Education [N N312 237435]
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Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) was isolated using a semi-preparative HPLC and its structure was confirmed by LC-ESI-MS. In LM of the investigated flaxseed, one molecule of caffeic acid corresponded with five molecules of p-coumaric acid and two molecules of ferulic acid. The presence of caffeic acid in the lignan molecule might be very beneficial due to its high antioxidant activity.
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