Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 120, Issue 2, Pages 519-530Publisher
SPRINGER
DOI: 10.1007/s11240-014-0618-x
Keywords
Encapsulation; Ocimum kilimandscharicum Guerke; Germplasm conservation; Genetic stability; RAPD analysis; ISSR analysis
Funding
- University Grants Commission (UGC), New Delhi
- University Grant Commission (UGC) New Delhi
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This article demonstrates the plantlet regeneration from alginate-encapsulated shoot tips of Ocimum kilimandscharicum Guerke. The best gel complexation for encapsulation of shoot tips was achieved using 3 % sodium alginate and 75 mM calcium chloride. Re-growth ability of encapsulated shoot tips explant was evaluated after storage at 4 and 25 A degrees C for 30, 45, 60, 75 and 90 days. Preservation at 25 A degrees C being significantly more resistant compared to that stored in 4 A degrees C. Maximum shoot re-growth (83.33 %) was recorded on 0.7 % agar solidified full strength MS basal medium supplemented with 1.0 mg/l BA. Micro shoots, recovered from encapsulated shoot tips (capsule) were best rooted on A1/2 strength MS medium containing 1.5 mg/l IBA. Complete plantlets (with shoot and root) were transferred to plastic pots containing soil: vermiculite (1:1) showing 79.53 % survival rate in field condition. The genetic stability of synseed-derived plantlets (following 4 and 25 A degrees C of storage) was assessed and compared with mother plant using two PCR based molecular markers namely random amplified polymorphic DNA and inter-simple sequence repeat. Both types of markers revealed high degree of monomorphism, similar to those of the mother plants.
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