Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 119, Issue 1, Pages 221-225Publisher
SPRINGER
DOI: 10.1007/s11240-014-0521-5
Keywords
Gracilaria edulis; Multiple shoot suspension culture; Sargassum wightii; Withanolides; Withania somnifera
Funding
- University Grants Commissions (UGC), Government of India of UGC-BSR (Basic Scientific Research Fellowship)
- Council of Scientific and Industrial Research (CSIR), Government of India of CSIR-SRF
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The influence of Gracilaria edulis and Sargassum wightii extracts was investigated for the production of biomass and withanolides in the multiple shoot suspension culture of Withania somnifera. Supplementation of 40 % G. edulis extract in MS liquid medium for 24 h exposure time in the culture recorded the highest biomass accumulation [62.4 g fresh weight and 17.82 g dry weight (DW)] and withanolides production (withanolide A 0.76 mg/g DW; withanolide B 1.66 mg/g DW; withaferin A 2.80 mg/g DW and withanone 2.42 mg/g DW) after 5 weeks of culture, which were 1.45-1.58-fold higher than control culture. This naturally available G. edulis extract-treated multiple shoot suspension culture protocol offers a potential alternative for the optimum production of biomass and withanolides utilizing shake-flasks.
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